Risultati per: L’alcool danneggia il DNA e aumenta il rischio di cancro.

Studio indaga sul volume esagerato

Nell’Ue 6 autorizzate, esame su linfoma e leucemia a cellule T

Implementazione del servizio, interesse delle altre Regioni

Implementazione del servizio, interesse delle altre Regioni

Objective
The presence of intestinal metaplasia (IM) is a risk factor for gastric cancer. However, it is still controversial whether IM itself is precancerous or paracancerous. Here, we aimed to explore the precancerous nature of IM by analysing epigenetic alterations.

Design
Genome-wide DNA methylation analysis was conducted by EPIC BeadArray using IM crypts isolated by Alcian blue staining. Chromatin immunoprecipitation sequencing for H3K27ac and single-cell assay for transposase-accessible chromatin by sequencing were conducted using IM mucosa. NOS2 was induced using Tet-on gene expression system in normal cells.

Results
IM crypts had a methylation profile unique from non-IM crypts, showing extensive DNA hypermethylation in promoter CpG islands, including those of tumour-suppressor genes. Also, the IM-specific methylation profile, namely epigenetic footprint, was present in a fraction of gastric cancers with a higher frequency than expected, and suggested to be associated with good overall survival. IM organoids had remarkably high NOS2 expression, and NOS2 induction in normal cells led to accelerated induction of aberrant DNA methylation, namely epigenetic instability, by increasing DNA methyltransferase activity. IM mucosa showed dynamic enhancer reprogramming, including the regions involved in higher NOS2 expression. NOS2 had open chromatin in IM cells but not in gastric cells, and IM cells had frequent closed chromatin of tumour-suppressor genes, indicating their methylation-silencing. NOS2 expression in IM-derived organoids was upregulated by interleukin-17A, a cytokine secreted by extracellular bacterial infection.

Conclusions
IM cells were considered to have a precancerous nature potentially with an increased chance of converting into cancer cells, and an accelerated DNA methylation induction due to abnormal NOS2 expression.

E anche le capacità mentali sono in pericolo 10 anni dopo

Studio, dalla bassa vitamina D alla solitudine

Helicobacter pylori, chi lo presenta ha probabilità più elevate

Nella Giornata internazionale indetta per prevenirle

Per lo stop degli anestesisti. Bloccato anche l’import-export alimentare

Sono circa 400mila gli italiani con obesità e scompenso cardiaco