Un vaccino sperimentale a mRNA ha potenziato gli effetti dell’immunoterapia […]
Search Results for: Nanotubi di DNA per terapie contro i tumori del cervello
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Malattia di Lyme, un biofilm rende batteri resistenti a terapie
Infezione trasmessa attraverso puntura di zecca
Malattia di Lyme, un biofilm rende batteri resistenti a terapie
Infezione trasmessa attraverso puntura di zecca
Puglia
I centri RISP (Rete Italiana Screening Polmonare) sono strutture sanitarie […]
Lazio
I centri RISP (Rete Italiana Screening Polmonare) sono strutture sanitarie […]
Campania
I centri RISP (Rete Italiana Screening Polmonare) sono strutture sanitarie […]
Lombardia
I centri RISP (Rete Italiana Screening Polmonare) sono strutture sanitarie […]
Linee guida per la sindrome genitourinaria della menopausa
Una nuova linea guida multi-organizzativa presenta 26 raccomandazioni per il […]
Tumori gastrointestinali in aumento tra gli under 50
Per nati 1990 rischio doppio a colon. Pesa cibo, alcol,poco moto
Crescono tumori gastrointestinali tra under-50,in 10 anni +14,8%
Per nati 1990 rischio doppio di cancro colon
Tumori cerebrali pediatrici, ok Aifa a combinazione terapeutica
Messo a punto da Int per il glioma diffuso della linea mediana
Fecondazione a 3 in Gb, 8 bebè nati senza malattie genetiche
Tecnica incrocia Dna genitori con quello di una donna donatrice
Sovrappeso e obesità invecchiano il cervello, negli uomini fino a 2 anni in più per chi ha troppi chili
Un peso eccessivo, in particolare lo stato di sovrappeso o di obesità, è associato a un invecchiamento accelerato del cervello e a una maggiore atrofia cerebrale, in particolare tra gli…
Sarcomi, andare in centri esperti aumenta possibilità di guarire
Luglio è il mese dedicato a questi tumori rari e aggressivi
TET3-Interacting LncRNA TILR Is Essential for DNA Hydroxymethylation–Mediated Neuroprotection After Ischemic Stroke
Stroke, Ahead of Print. BACKGROUND:Epigenetic modifications 5-methylcytosine and 5-hydroxymethylcytosine in DNA regulate neuronal survival under ischemic stress. We previously showed that TET3 (ten-eleven translocation 3)–mediated 5-methylcytosine to 5-hydroxymethylcytosine conversion induces neuroprotective gene transcription after stroke. As TET3 neuronal isoform lacks the DNA-binding domain, how TET3 drives 5-hydroxymethylcytosine–mediated transcriptional induction in the ischemic brain remains unclear. Long noncoding RNAs (lncRNAs) act as structural scaffolds to recruit chromatin-modifying proteins and other RNAs to specific genomic loci. However, whether TET3 requires an lncRNA to drive DNA hydroxymethylation in the ischemic brain is unknown.METHODS:Adult male and female mice were subjected to transient middle cerebral artery occlusion. TET3-bound lncRNAs were immunoprecipitated from peri-infarct cortex, and TILR (TET3-interacting lncRNA; AK020504) identified was inhibited with small interfering RNA injected at 5 minutes of reperfusion. Ascorbate was administered at 30 minutes of reperfusion to induce TET3 activity. Poststroke DNA hydroxymethylation was assessed with hydroxymethylation DNA immunoprecipitation sequencing, and sensorimotor deficits, and infarct volume were evaluated between days 1 and 7 of reperfusion.RESULTS:TILR binds to TET3 with high affinity and was significantly upregulated in the peri-infarct cortex at 12 hours of reperfusion. Knockdown of TILR increased the infarct volume and reduced the motor function recovery after transient middle cerebral artery occlusion, in a TET3-dependent manner. On contrary, TET3 activation by ascorbate decreased brain damage and improved motor function recovery after ischemia. However, ascorbate-induced postischemic protection was abrogated by TILR knockdown. Genome-wide profiling showed that ascorbate increases the number of differentially hydroxymethylated regions in the poststroke genome, a neuroprotective effect that is reversed by TILR knockdown. Moreover, TILR inhibition significantly reduced the DNA hydroxymethylation in the intergenic regions associated with enhancers, super enhancers, and the promoters of other lncRNAs, microRNAs, and PIWI-interacting RNAs.CONCLUSIONS:These findings highlight the essential role of TILR in TET3-mediated 5-hydroxymethylcytosine-dependent epigenetic reprogramming in the ischemic brain.
Advances in Preventing Transmission of Mitochondrial DNA Diseases
New England Journal of Medicine, Ahead of Print.