Circulation, Volume 150, Issue Suppl_1, Page A4144098-A4144098, November 12, 2024. Background:Reduced level of IgG autoantibodies specific for the apoB-100 peptide P210 is associated with increased risk for myocardial infarction (MI). However, the underlying mechanism for reduced P210 IgG levels in patients at risk of MI is unknown.Methods:We evaluated the baseline P210 IgG and immune complex (P210 IgG-IC) levels using ELISA of plasma samples of a sub-cohort of volunteers having coronary artery calcium scans (CACS) as part of the EISNER (Early Identification of Subclinical Atherosclerosis by Noninvasive Imaging Research) trial. The sub-cohort selected were those that did not have MI (No MI, N=90) and those that had a future MI (FMI, N=23) during follow-up (13.2±2.1 years). We also evaluated plasma samples collected from patients admitted to Cedars-Sinai for acute coronary syndrome (ACS, N=15). ELISA for P210 IgG and ICs were standardized against a pool of healthy control plasma and expressed as adjusted optical density (Adj OD). Flow cytometry evaluation of CXCR5+ T follicular helper (Tfh) cells that regulate IgG levels was performed in peripheral blood mononuclear cells (PBMCs) from ACS patients (N=8) in response to in vitro stimulation with P210. Specimen and data collection were IRB approved.Results:P210 IgG was significantly lower in FMI patients compared to No MI and ACS patients (FMI=0.6±0.6 vs No MI=1.5±1.6 and ACS=1.4±0.9 Adj OD; P
Abstract 4144098: Mechanisms Of Reduced IgG Autoantibodies Specific to the ApoB-100 Peptide P210 Associated With Increased Risk For Myocardial Infarction
Read More →
Novembre 2024
